STUDIES ON MECHANISM OF THE STREPTOMYCIN REACTION
نویسندگان
چکیده
منابع مشابه
Counter-current distribution studies on streptomycin; the tautomerism of streptomycin.
The simplicity and reproducibility which characterize the conversion of streptomycin to malt01 (1) by heating in dilute alkali have made this reaction the basis of a frequently used analytical method for streptomycin (2). When used in these laboratories as a method of assaying streptomycin preparations, however, the malt01 reaction consistently gave lower yields per unit of potency with pure st...
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The stereospecificity of the chicken heart mitochondrial malate dehydrogenase as well as the ability of this enzyme to form various abortive complexes has been further investigated. The enzyme was found to be specific for the A-hydrogen of NADH. Complex formation of the enzyme with oxalacetate and oxidized coenzymes is pH-dependent and is promoted at alkaline pH values. The enol form of oxalace...
متن کاملKinetic studies on the mechanism of the malate dehydrogenase reaction.
This work is a kinetic investigation of the reaction mechanism of malate dehydrogenase, prepared from washed mince of whole bovine heart by a variation on previous methods. The forward and reverse reactions catalyzed by this enzyme have been studied at pH 8.0 in the presence and in the absence of one product at a time, with the use of a recording fluorometer to measure changes in the concentrat...
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1; Heat extracts of mature mycelia of streptomycin-producing strains of Sfreptomyces contain two compounds which, when incubated with arginine-guanidino-‘*C plus a crude enzyme preparation from S. griseus ATCC 12475 containing a&nine: X amidinotrausferase, give products detected as radioactive Peaks I and II. Heat extracts from S. bluensis var. bluensis, which synthesizes bluensomycin, a monogu...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1958
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.75.1.11-15.1958